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. 2022 Nov 24;13(1):342–354. doi: 10.1080/21645698.2022.2141014

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Strategy of tissue culture-independent in planta transformation in Mango (Mangifera indica L.). (a) Seeds without seed coat sown in potting mixture allowed to germinate; (b) Hook shaped plumule emerging out of seed indicating its growth; (c) Stage of seedling suitable for pricking; (d) Pricking of the emerging plumule with an insulin needle; (e) Agrobacterium-mediated transformation of seedlings; (f) Planting of transformed seedlings onto polybag and recovery of primary transformants; (g) Recovered plants in growth chamber (h) Plants transferred to glasshouse; and (i) established plants.