Skip to main content
. 2022 Nov 24;13(1):342–354. doi: 10.1080/21645698.2022.2141014

Figure 5.

Figure 5.

Molecular analysis of primary transformants harboring pCAMBIA1302 GFP::hptII. (a. i–ii; b. i–ii) PCR analysis for the amplification of GFP gene (571 bp) and hptII (700 bp) gene fragments. Lane M- 1Kb marker, Lane B- water blank, Lane WT- wild-type DNA, Lane P- plasmid (25 ng). Lanes 1 F-12 F and 13 F-24 F primary transformants. (c.) PCR analysis for the amplification of 438 bp Agrobactrium-specific VirD1 gene in transgenic Mango plants. Lane M- 1 Kb marker (Thermo scientific), Lane B- water blank, Lane WT- wild type, Lanes 1 F, 5 F, 9 F, 15 F, 24 F- transgenic plants, Lane P- binary vector, +ve is DNA from Agrobacterium strain EHA 105 (d) sqRT-PCR analyses for the assessment of transgene transcripts. (i) 124 bp GFP, (ii) 130 bp hptII and (iii) 134 bp MiACT1. Lane B- water blank, Lane WT- wild type, Lanes 1 F, 5 F, 9 F, 15 F, 24 F- transgenic plants, Lane P- binary vector. (e) Genomic Southern analysis of transgenic plants probed with DIG-labeled 571 bp GFP gene fragment, Lane L- Lambda HindIII DNA digest, Lane WT- untransformed wild type, Lanes 1 F, 15 F- transgenic Mango, P- linearized plasmid of pCAMBIA1302 10 pg.