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. 2022 Nov 24;13(1):342–354. doi: 10.1080/21645698.2022.2141014

Figure 6.

Figure 6.

Molecular analysis of primary transformants harboring pCAMBIA 2301 GUS::nptII. (a) PCR analysis for the amplification of (i) GUS gene (1000 bp) and (ii) nptII (750 bp) fragments. Lane M- 1 kb marker, Lane B- water blank, Lane WT- wild-type DNA, Lanes 1 U-12 U- are primary transformants, Lane P- plasmid (25 ng). (b) sqRT-PCR amplified products on 1.5% w/v agarose gel of (i) 122 bp GUS, (ii) 67bp nptII and (iii) 134 bp MiACT1. Lane B- water blank, Lane WT- wild type, Lanes 1 U, 4 U, 7 U, 13 U, 15 U- transgenic plants, Lane P- binary vector. (c) Genomic Southern analysis of transgenic plants probed with DIG-labeled 750 bp nptII gene fragment, Lane L- Lambda HindIII DNA digest, Lane WT- wild type, Lane 1 U- transgenic Mango, Lane P- linearized plasmid of pCAMBIA2301 (10 pg).