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. 2022 Nov 25;13(1):2042–2058. doi: 10.1080/21505594.2022.2149973

Figure 4.

Figure 4.

Smh1 is a histone-deacetylase and is active in the nucleus of the host cell where it targets histone 3 and 4. (a) THP-1Smh1 cells were stimulated with PMA (80 nM) for 72 h. Afterwards, the cytoplasmic and nucleic fractions were separated. Western Blot was performed against the Smh1-fused HA-tag. Lamin a was used as housekeeper for the nuclear fraction and α1c Tubulin for the cytosolic fraction. One representative blot of 3 is shown. The relative quantification of Smh1 is normalized to the cytosolic fraction. A paired t-test was performed. Data are shown as mean + SEM of three independent biological replicates. *p ≤ 0.05. THP-1Smh1 and corresponding control cells (THP-1Ctrl) were stimulated with PMA (80 nM) for 72 h. Function of Smh1 as a histone-deacetylase was confirmed by examination of histone 3 and 4 acetylation (H3ac and H4ac) by Western Blot (b and c). One representative blot of three independent biological replicates is shown. Relative quantification of H3ac and H4ac in comparison to actin is normalized to Ctrl. Unpaired t-test was performed, and data are shown as mean + SEM of three independent biological replicates. *p ≤ 0.05; **p ≤ 0.01.