Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 30;226(11):1959–1963. doi: 10.1093/infdis/jiac272

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 1. — Dengue virus (DENV)-2 antibody depletion and specificity of neutralizing antibody (nAb) response to TAK-003. (A) Schematic of DENV-2 antibody depletion, and log reporter virus particle (RVP) half-maximal effective concentration (EC₅₀) titers for (B) DENV-1, (C) DENV-3, and (D) DENV-4 after DENV-2 antibody depletion. Sera from baseline-seronegative individuals vaccinated with TAK-003 (n = 71) collected 30 days after second vaccination from studies DEN-301 (red dots) and DEN-304 (blue dots) were depleted with mock antigen (x-axis) and DENV-2 antigen (y-axis) bound to magnetic beads, and their neutralization titers were measured by dengue RVP assay. Log₁₀ EC₅₀ represents the dilution factor required to neutralize 50% of RVPs. Samples where depletion of anti-DENV-2 antibodies leads to complete loss of detectable nAbs against DENV-1, 3 and/or 4 (seen as log₁₀ EC₅₀ < limit of detection (LOD) and represented by the open circles) contain a high proportion of cross-reactive antibodies. Samples where DENV-1, -3 and/or -4 titer remain > LOD postdepletion with DENV-2 (represented with closed circles) contain type-specific antibodies. HD, high dose.