Figure 5.

Flow cytometry results indicating loss of bulk lipids and retention of PEGylated lipids. (A) AF488 fluorescence intensity was used as an analog for relative shell concentration of PEGylated lipids. There was no significant difference between ADV/RC and control groups (N = 5, p > 0.05), indicating that the PEGylated lipids were fully retained. (B) DiI fluorescence intensity was used as an analog for relative shell concentration of bulk lipids (DSPC). The median fluorescence intensity was significantly lower in the ADV/RC group as compared to controls (N = 5, p < 0.05). These results indicated that 20% of the bulk lipids were shed during ADV/RC. For both parts A and B, two representative example histograms, illustrating the distribution of droplet fluorescence intensities, are plotted alongside the associated median fluorescence intensity values (dashed vertical lines). (C) Median fluorescence intensity values are plotted, averaged for each group (N = 5). Note that values should not be directly compared between AF488 and DiI, as these were two independent droplet populations which used distinct sets of emission filters. PEGylated lipids were retained whereas 20% of the bulk lipids were shed during ADV/RC. α: p < 0.05.