Droplets retain selective binding capabilities following vaporization
and recondensation. (A) A plot of the number of droplets bound within and
outside of the
αvβ3
coated region, normalized to the number bound outside of the
αvβ3
coated region, is shown. No significant differences were observed in the RAD
negative control group, indicating that any enhancement of binding efficacy in
the two RGD groups was due to specific receptor–ligand interactions. Both
control and ADV/RC RGD groups exhibited significantly more droplets bound within
the αvβ3
coated region, indicating both groups selectively bound
αvβ3.
α: p < 0.05, RGD +
αvβ3
vs –
αvβ3.
β: p < 0.05, RGDADV +
αvβ3
vs –
αvβ3.
Representative images of RAD, RGD, and RGDADV groups are shown in the –
αvβ3
(B–D) and +
αvβ3
(E–F) regions. RGDADV = RGD droplets that underwent ADV/RC.