Fig 7. Rap1 knockin mice exhibit neurological changes in a sex-dependent manner.

(A) Wire-hang test showing maximum latency of mice to fall, n = 19 WT (9 females and 10 males) and n = 24 Rap1^KI/KI (14 females and 10 males) mice. P values in WT vs. Rap1^KI/KI females and /or males were shown. (B) buried food test. n = 19 WT (10 females and 9 males), n = 19 Rap1^KI/KI (12 females and 7 males). P values in WT vs. Rap1^KI/KI females and/or males were shown. (C) open-field test/distance traveled in 15 mins. n = 21 WT (11 females and 10 males) and n = 24 Rap1^KI/KI (14 females and 10 males). p = 0.128 (WT vs. Rap1^KI/KI). (D) open-field test/time in the center out of 15 mins. p = 0.003 (genotype/sex interaction). (E) zero-maze test, n = 14 WT (8 females and 5 males) and n = 11 Rap1^KI/KI (5 females and 6 males) mice. p = 0.054 (genotype/sex interaction). (F) Data for behavioral tests displayed as % WT control (set to 100%). Behavior results were analyzed in R software with 2-way ANOVA using genotype and sex as factors, with covariates of wave and body weight included where appropriate. All data are mean ± SEM.