TABLE 1.
Condition | Description | % Apoptosis at 18 h
for staining with:
|
|
---|---|---|---|
PIa | MC540b | ||
Medium | Control | 9.3 | 1.5 |
35000c | Parent | 14.0 | 24.0 |
35000HP-RSM1 | Hemolysin mutant | 13.3 | 23.8 |
35000.303 | cdtC mutant | 2.4 | 2.2 |
35000.303(pLS88) | cdtC mutant and vector | 5.4 | 2.8 |
35000.303(pJL300-C) | Complemented cdtC mutant | 14.5 | 30.8 |
Values are representative of four experiments. The FTWC concentration was 1 μg/ml.
Values are representative of five experiments. The FTWC concentration was 10 μg/ml.
35000 and 35000HP gave similar results; for simplicity, only data obtained with 35000 are shown.