Fig. 1.

Generation of the R1D3 antibody based on mAb 1D3. (A) Predicted polypeptide sequences for mAb 1D3 H and L chains. Colors indicate leader (light blue), variable (red), and constant regions (green). * denotes the terminal carboxy group. (B) Cell production of R1D3 antibody. Time course of the production of soluble R1D3 in transfected HEK293 cells (1/50 dilution of the culture media) analyzed by indirect ELISA with adsorbed native cyt c (0.5 µM). Plates were developed and absorbance at 450 nm was recorded. (C) SDS–PAGE analysis of mAb R1D3. SDS–PAGE (10%) of purified mAb 1D3 (lane 1 and 3, 20 µg) and R1D3 (lane 2 and 4, 5 µg) expressed in HEK293 cells under nonreducing and reducing conditions (dithiothreitol, 1 mM) following Coomassie blue stain.