Fig. 7.

mAb 1D3 detection of an alternative conformation of cyt c following H₂O₂ treatment of Vero cells. Cells were treated with H₂O₂ (0.05% in PBS) and incubated for 2 h in DMEM media without FBS and fixed. Cells were then probed with mAb 1D3 and R1D3 followed by antimouse-FITC (green) conjugate antibody and analyzed by confocal microscopy. Horizontal scale bars indicate 10 µm.