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. Author manuscript; available in PMC: 2022 Nov 28.
Published in final edited form as: Nat Cell Biol. 2022 Jun 30;24(7):1165–1176. doi: 10.1038/s41556-022-00942-8

Extended Data Fig. 4. Characterization of in vitro generated exhausted CD8 T cells.

Extended Data Fig. 4.

a,b, Flow cytometry analysis of the indicated ICRs, TOX, T-bet, and TCF1 (a) or effector-related molecules (b) in wildtype OT1 CD8 T cells cultured in vitro under mock or OVA257-264-stimulated effector or exhaustion conditions. c-g, Immunoblot analysis of Dapl1 knockdown (c) and flow cytometry analysis of the expression of PD1+ (d), Tim3+ (e), TOX (f), and TCF1 (g) in human CD8 T cells transduced with a control (Ctrl) shRNA or a Dapl1-specific shRNA and cultured under unexhaustion(cultured with 50 U/ml hIL-2) and exhaustion (three round of stimulation with anti-CD3/anti-CD28 Dynabeads) conditions. Data are representative of three independent experiments.