Extended Data Fig. 5. Dapl1 has a cell-intrinsic function in regulating CD8 T cell functions.

a, Schematic of experimental design. b-g Flow cytometry analysis of the frequency of splenic (Spl) or TIL CD45.1⁺CD8⁺ or CD45.2⁺CD8⁺ T cells (b), Granzyme B-, IFN-γ-, TNF-α-producing or Ki67⁺ CD8 TILs (c), PD1⁺,Tim3⁺, Lag3⁺ or TIGIT⁺ CD8 TILs (d), TCF1⁺Tim3⁻, TCF1⁺Tim3⁺ and TCF1⁻Tim3⁺ subsets in gated CD8⁺PD1⁺ TILs (e), Granzyme B-, IFN-γ-, TNF-α-producing cells in gated PD1⁺Tim3⁺ CD8 TILs (f), or Ki67⁺CD8⁺ cells in gated PD1⁺Tim3⁺ CD8⁺ TILs (g) of B16-OVA tumor-bearing Rag1 KO recipient mice adoptively transferred with a mixture of BM cells derived from B6.SJL mice (CD45.1⁺) and Dapl1 KO (CD45.2⁺) or wildtype mice (CD45.2⁺). Data are presented as representative FACS plots and summary graphs. n = 5 chimeric mice. h-l, Flow cytometry analysis of the frequency of CD45.1⁺CD8⁺ or CD45.2⁺CD8⁺ T cells (h), H2Db/gp33-41 Tetramer⁺ CD8⁺ T cells (i), IL-2-, IFN-γ-, Granzyme B-, TNF-α-, or Ki67⁺-producing CD8 T cells (j), PD1⁺, Tim3⁺, Lag3⁺, TIGIT⁺, PD1⁺Tim3⁺ CD8 T cells or TCF1⁺Tim3⁻, TCF1⁺Tim3⁺, TCF1⁻Tim3⁺ subsets (k), and IFN-γ-, IL-2-, TNF-α- or Granzyme B-producing CD8 T cells (l), gated on PD1⁺Tim3⁺ CD8⁺ T cells in the spleen of Rag1 KO recipient mice adoptively transferred with a mixture of BM cells derived from B6.SJL mice (CD45.1⁺) and Dapl1 KO (CD45.2⁺) or wildtype (CD45.2⁺) mice, infected i.v. with LCMV clone 13 for 30 days. Data are presented as representative FACS plots and summary graphs. n = 5 chimeric mice. Summary data are shown as the mean±s.d. with P values determined using a two-tailed unpaired Student’s t-test (b-l). ns, not significant.