Extended Data Fig. 6. Dapl1 has a cell-intrinsic role in regulating CD8 T cell responses to LM-OVA infection and cancer.

a, Schematic of experimental design for generating mixed bone marrow chimeric mice and LM-OVA infection. b,c, Flow cytometry analysis of the frequency of IFN-γ- or Granzyme B-producing CD8 effector T cells (b) and TCF1⁺ CD8⁺, TCF1⁻ CD8⁺ cells (c) in the spleen of Rag1 KO recipient mice adoptively transferred (for 6 week) with a mixture of BM cells derived from B6.SJL mice (CD45.1⁺) and Dapl1 KO (CD45.2⁺) or wildtype (WT, CD45.2⁺) mice, infected i.v. with LM-OVA for 7 days. Data are presented as representative FACS plots and summary graphs. n = 5 chimeric mice. d-k, Schematic of experimental design (d) and flow cytometry analysis of the frequency of wildtype (CD45.1⁺CD45.2⁺) and Dapl1 KO (CD45.2⁺) CD8 T cells (e), Ki67⁺ CD8 T cells (f), effector CD8 T cells (g), Tim3⁺ or PD1⁺ CD8 T cells (h), TCF1⁺Tim3⁻, TCF1⁺Tim3⁺ and TCF1⁻Tim3⁺ subsets in gated PD1⁺ CD8 TILs (i), Ki67⁺ cells in gated PD1⁺Tim3⁺ CD8 T cells (j), and Granzyme B-, IFN-γ-, or TNF-α-producing cells in gated PD1⁺Tim3⁺ CD8 T cells (k) in the spleen (Spl) or the tumor (TIL) of B16F10 tumor-bearing B6.SJL mice (CD45.1⁺) adoptively transferred with a mixture (1:1) of wildtype (CD45.1⁺CD45.2⁺) and Dapl1 KO (CD45.2⁺) Pmel1 CD8 T cells. Data are presented as representative FACS plots and summary graphs. n =5 recipient mice. Summary data are shown as the mean ± s.d. with P values determined using a two-tailed unpaired Student’s t-test (b, c, e-k). ns, not significant.