Fig 4. Tolerance can mask persister determinants during macrophage infection.

(A-B) CFU enumeration of survival of the WT, ΔhisG, shpAB1, shpAB1ΔhisG, ΔrecA and ΔrecAΔhisG Salmonella strains in BMDMs treated with cefotaxime and in absence of histidine. (C) Representative flow cytometry contour (left) and histogram plots (right) of the shpAB1, shpAB1ΔhisG, ΔrecA and ΔrecAΔhisG strains extracted from infected BMDM after 16 h of gentamicin. G, growers and NG, non-growers. (D) Quantification of the non-grower fraction for each strain represented in panel C. Data represent the mean and SD of three biological repeats. Data were compared using one way ANOVA with Tukey’s multiple-comparison test, ***p<0.001; NS, not significant. Of note, WT and ΔhisG data are the same on panel A and B as all experiments were conducted in parallel. Auxotrophic strains are depicted in orange, prototrophic in turquoise.