Figure 3. Pseudotemporal analysis of pig thymocyte development.
(A) Pseudotime trajectory created by Monocle 3 using clusters 0–14 from Figure 1A.
(B) The same UMAP plot showing classical stage-specific markers of thymocyte development.
(C) Heatmap of 16 gene modules whose expression varied across pseudotime between clusters.
(D) UMAP plots showing the expression profiles of select genes from modules 1–13. See Table S4 for a complete list of module genes. Asterisks indicate non-annotated genes (described in Table S13)