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. Author manuscript; available in PMC: 2022 Nov 28.
Published in final edited form as: Cell Rep. 2022 Jul 5;40(1):111050. doi: 10.1016/j.celrep.2022.111050

Figure 5. scRNA-seq analysis of porcine thymic iNKT cells.

Figure 5.

(A) Flow cytometry showing thymic phycoerythrin (PE)-conjugated mouse (m)CD1d tetramer+ cells before (left panel) and after (center panel) isolation with magnetic beads and FACS. Purity was confirmed by co-staining with allophycocyanin (APC)-conjugated PBS57-loaded or unloaded mCD1d tetramers (right panel).

(B) UMAP visualization of iNKT thymocyte clusters identified using the graph-based Louvain algorithm at a resolution of 0.5.

(C) Dot plot showing the Z-scored mean expression of selected genes encoding key transcription factors and thymocyte differentiation markers for each cluster.

(D) Heatmap showing row-scaled mean expression of the five highest differentially expressed transcription factors per cluster.

(E) Integrative analysis of iNKT cells and whole thymocytes (excluding B cells) from the same pigs. Asterisks indicate non-annotated genes (described in Table S13).