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. 2022 Nov 28;11:e78754. doi: 10.7554/eLife.78754

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© 2022, Chang et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 5. — (A) A schematic representation of the offspring produced when △3.8 heterozygous female and hIC1/+ male mice are mated is depicted. △3.8/hIC1 embryos are expected to show activation of maternal Igf2 expression as well as paternal H19 expression. (B) Relative fetal weights of wild-type, △3.8/+, +/hIC1, and △3.8/hIC1 embryos at E11.5 and E17.5, normalized to the average body weight of wild-type littermates (mean ± SD). 2 E11.5 litters and 10 E17.5 litters are presented. (C) Representative cross-sections of E17.5 △3.8/+ and E18.5 △3.8/hIC1 embryonic hearts with ventricular septal defects (VSDs; yellow arrows), stained with hematoxylin and eosin. Sections from wild-type littermates are shown together for comparison. The E17.5 wild-type sample is male, and the rest are female. Scale bars = 500 µm. (D) Relative total expression of H19 and Igf2 in E17.5 wild-type, △3.8/+, +/hIC1, and △3.8/hIC1 hearts (mean ± SD). (B and D) Each data point represents an individual conceptus from different litters. One-way ANOVA with Tukey’s multiple comparisons test was used with *p<0.05, ***p<0.001, ****p<0.0001, and ns = not significant.

Figure 5—figure supplement 1. — (A) A schematic representation of the offspring produced when △3.8 heterozygous female and hIC1/+ male mice are mated is depicted. △3.8/hIC1 embryos are expected to show activation of maternal Igf2 expression as well as paternal H19 expression. (B) Relative fetal weights of wild-type, △3.8/+, +/hIC1, and △3.8/hIC1 embryos at E11.5 and E17.5, normalized to the average body weight of wild-type littermates (mean ± SD). 2 E11.5 litters and 10 E17.5 litters are presented. (C) Representative cross-sections of E17.5 △3.8/+ and E18.5 △3.8/hIC1 embryonic hearts with ventricular septal defects (VSDs; yellow arrows), stained with hematoxylin and eosin. Sections from wild-type littermates are shown together for comparison. The E17.5 wild-type sample is male, and the rest are female. Scale bars = 500 µm. (D) Relative total expression of H19 and Igf2 in E17.5 wild-type, △3.8/+, +/hIC1, and △3.8/hIC1 hearts (mean ± SD). (B and D) Each data point represents an individual conceptus from different litters. One-way ANOVA with Tukey’s multiple comparisons test was used with *p<0.05, ***p<0.001, ****p<0.0001, and ns = not significant.