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. 2022 Oct 28;89(6):e60–e68. doi: 10.1097/SAP.0000000000003316

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Copyright © 2022 The Author(s). Published by Wolters Kluwer Health, Inc.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

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FACS analysis of ASCs. A, Expression of surface stem cell markers, positive (CD90, CD73, and CD105) and negative (CD34 and CD45) detected by flow cytometric analysis of cultured ASCs. Data show a representative set of dot plot from subject 5 compared with the positive control (hMSCs) and negative control (unstained hMSCs) (B, Summary of the immunophenotypic characteristics of ASCs. For the enzymatic processing method, data are representative of the analysis of 9 individuals, and for the nonenzymatic processing method, data are representative of the analysis of 6 individuals compared with the positive control (hMSCs) and negative control (PC-3 cells). ***Significant difference between enzymatic and negative control was found for CD90 and CD73 (n = 9, P < 0.001). A significant difference between nonenzymatic and negative control was found for CD90 and CD73 (n = 6, P < 0.001).