High-resolution deconvolution immunofluorescence microscopy of mucin granules in mouse airways stimulated with cytokines. (A) Deconvolved image of a mouse airway secretory cell after IL-1β challenge and staining with antibodies against MUC5AC (red) and MUC5B (green). Scale bar, 5 μm. (B) Higher magnification of mucin granules from box in A containing both mucins. Scale bar, 1 μm. (C) Image of a mouse airway secretory cell after IL-13 challenge and stained with the same antibodies. Scale bar, 5 μm. (D) Higher magnification of mucin granules from box in C containing both mucins. Scale bar, 1 μm. MUC5AC = mucin 5AC, oligomeric gel-forming; MUC5B = mucin 5B, oligomeric gel-forming.