FIG. 1.
(A) His-tagged intimin constructs used in this study. Full-length intimin contains 934 amino acids. The RIHisEae, His-IntN1/3, and His-IntN2/3 proteins lack the N-terminal 35 amino acids of the native protein (47). Each of the intimin fragments has a six-histidine tag at the amino terminus. The construction of the plasmids encoding these proteins is described in Materials and Methods; the plasmid encoding RIHisEae was described previously (47). (B) Coomassie-stained SDS-PAGE gel of His-tagged intimin fragments designed for this study after purification by nickel affinity chromatography. Lane 1, His-IntN1/3; lane 2, His-IntMid1/3; lane 3, His-IntN2/3; lane 4, His-IntC1/3; lane 5, His-IntC1/2. The stained gel was scanned with a Hewlett-Packard ScanJet 4C scanner, and the figure was labeled with Adobe Photoshop version 5.0.2 for Macintosh.