FIG. 5.

(A) Effect of BM-1 on R. rickettsii-induced expression of TF mRNA. Levels of TF mRNA (398-bp product) and GAPDH mRNA (588-bp product) were analyzed in total RNA samples prepared from uninfected EC (Control), uninfected EC in the presence of BM-1 (Control + BM-1), R. rickettsii-infected EC (RR), and infected EC in the presence of BM-1 (RR + BM-1) by RT-PCR analysis with specific primer pairs. The amplification products generated after 30 amplification cycles are shown. (B) Effect of calphostin C (CC) on R. rickettsii-induced expression of TF mRNA. Levels of TF mRNA and GAPDH mRNA were analyzed in total RNA preparations from R. rickettsii-infected EC (RR), infected EC in the presence of CC (RR + CC), uninfected EC (Control), and uninfected EC in the presence of CC (Control + CC) by RT-PCR analysis with the primer sequences described in Materials and Methods.