Figure 6.

Diabetes did not impact ex vivo urothelial permeability to Evans blue at the 30-wk (underactive detrusor) time point. Isolated mucosal layer preparations were inflated with Evans blue dye containing buffer and submerged in Krebs solution, as described in methods. After 30 min, the solutions were collected and quantified spectrophotometrically against a standard curve. Evans blue in these solutions that had permeated through the mucosa was indicative of barrier damage. A and B: at 30-wk time points, Evans blue permeability was not statistically different between nondiabetic and diabetic mice in the presence or absence of the NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) gene. n = 7, 8, 4, and 6 animals, respectively. There were no statistically significant differences (Student’s t test).