Figure 10.

No changes in uroplakin and tight junction protein expression are observed at the 30-wk (underactive detrusor) time point. Western blots were performed using standard techniques described in methods to determine protein expression of uroplakin subunit UP1b and zona occludens 1 (ZO1) in mucosal layer lysates from all four groups of 30-wk mice. A: representative images showing fluorescent labeled UP1b, ZO1, and GAPDH proteins from all four groups of mice and the respective molecular weights of each protein. B and C: regardless of NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) status, diabetes did not impact UP1b expression in 30-wk diabetic mice. D and E: ZO1 protein expression was also not affected by diabetes at 30 wk either in the presence or absence of NLRP3. n = 6 animals per group.