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. 2022 Nov 21;2022:6477132. doi: 10.1155/2022/6477132

Figure 5.

Figure 5

Effects of THC and CBN on CCA cell signaling. (a) The human phosphokinase array detected phosphorylated proteins in the control group, in HuCCT1 cells treated with 20 μM of THC and in cells treated with 20 μM of CBN after an 18 h incubation. Corresponding signals: (i) GSK-3α/β (phosphorylated at Ser21/9), (ii) ERK1/2 (phosphorylated at Thr202/Tyr204), and (iii) AKT (phosphorylated at Ser473). (b) Relative changes in phosphorylated kinase proteins between THC- or CBN-treated HuCCT1 cells and untreated HuCCT1 cells were demonstrated. (c) Selected phosphorylated kinase proteins in HuCCT1 cells after 10 μM to 20 μM THC or CBN treatment for 18 h were determined by western blotting. At a concentration of 20 μM, THC and CBN inhibited the phosphorylation of AKT, GSK-3α/β, and ERK1/2. β-Actin was used as a loading control. (d) The relative levels of phosphorylation of AKT, GSK-3α/β, and ERK1/2 from western blot analyses were normalized to β-actin level (∗P < 0.01 versus control).