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. 1999 Dec;67(12):6424–6433. doi: 10.1128/iai.67.12.6424-6433.1999

TABLE 4.

Stability within CVD 908-htrA of ori15A replicons, containing plasmid maintenance systems of increasing complexity, grown without selection and in the presence of increasing osmolaritya

Strain or plasmid Stability result with NaCl concn:
50 mM
150 mM
300 mM
OD600b % Fluorescing bacteria Mean fluorescence intensityc OD600 % Fluorescing bacteria Mean fluorescence intensityc OD600 % Fluorescing bacteria Mean fluorescence intensityc
Strain
 CVD 908-htrA 0.98 100 0.6 1.11 100 0.6 1.12 100 0.6
Plasmids
 pGEN91 1.00 13.2 28.6 1.17 11.4 42.9 1.26 10.9 65.5
 pGEN111 1.26 47.4 51.8 1.17 28.9 93.6 1.12 42.4 65.1
 pGEN121 1.01 80.5 53.3 1.20 73.8 74.0 1.15 56.7 105.3
 pGEN193 1.11 71.4 50.9 1.24 65.2 64.7 1.22 53.7 90.8
 pGEN222 1.01 96.8 52.1 1.28 93.3 67.8 1.13 95.3 89.2
a

These data are represented by histograms in Fig. 2

b

OD600, optical density at 600 nm. 

c

All standard error values are <0.6.