TABLE 4.
Stability within CVD 908-htrA of ori15A replicons, containing plasmid maintenance systems of increasing complexity, grown without selection and in the presence of increasing osmolaritya
| Strain or plasmid | Stability result with NaCl concn:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 50 mM
|
150 mM
|
300 mM
|
|||||||
| OD600b | % Fluorescing bacteria | Mean fluorescence intensityc | OD600 | % Fluorescing bacteria | Mean fluorescence intensityc | OD600 | % Fluorescing bacteria | Mean fluorescence intensityc | |
| Strain | |||||||||
| CVD 908-htrA | 0.98 | 100 | 0.6 | 1.11 | 100 | 0.6 | 1.12 | 100 | 0.6 |
| Plasmids | |||||||||
| pGEN91 | 1.00 | 13.2 | 28.6 | 1.17 | 11.4 | 42.9 | 1.26 | 10.9 | 65.5 |
| pGEN111 | 1.26 | 47.4 | 51.8 | 1.17 | 28.9 | 93.6 | 1.12 | 42.4 | 65.1 |
| pGEN121 | 1.01 | 80.5 | 53.3 | 1.20 | 73.8 | 74.0 | 1.15 | 56.7 | 105.3 |
| pGEN193 | 1.11 | 71.4 | 50.9 | 1.24 | 65.2 | 64.7 | 1.22 | 53.7 | 90.8 |
| pGEN222 | 1.01 | 96.8 | 52.1 | 1.28 | 93.3 | 67.8 | 1.13 | 95.3 | 89.2 |
These data are represented by histograms in Fig. 2.
OD600, optical density at 600 nm.
All standard error values are <0.6.