Fig. 3.

hUC-MSCs inhibited apoptosis of SU5416-treated lungs. (A) Re-presentative results of TUNEL assay using lung sections chosen from each group. There was no significant difference between the Control (CON) group and the Control＋MSC (CON＋MSC) group. Increased TUNEL-positive cells were observed in the group treated with SU5416 (SU). hUC-MSCs reduced TUNEL-positive cells (SU＋MSC). Magnification, ×400; scale bar, 50 μm. (B) Quantitative results of TUNEL assay showing apoptosis index of the four groups. Data are presented as mean±SEM. ****p<0.0001 compared with the Control (CON) group, ^####p<0.0001 compared with the SU5416 (SU) group. n=3. (C) Representative results of active caspase-3 and PCNA as determined by western blotting. hUC-MSCs inhibited increased levels of active caspase-3 but not affect proliferation in the SU5416-treated models.