Table 1.
Gene | Forward primer sequence (5’–3’) | Reverse primer sequence (5’–3’) |
---|---|---|
β-actin | CTACCTCATGAAGATCCTGACC | CACAGCTTCTCTTTGATGTCAC |
TNF-α | ATGTCTCAGCCTCTTCTCATTC | GCTTGTCACTCGAATTTTGAGA |
IL-6 | TCTGGAGCCCACCAAGAACGATAG | GTCACCAGCATCAGTCCCAAGAAG |
Runx2 | CCTTCAAGGTTGTAGCCCTC | GGAGTAGTTCTCATCATTCCCG |
ALP | AACCCAGACACAAGCATTCC | CCAGCAAGAAGAAGCCTTTG |
OCN | GGACCATCTTTCTGCTCACTCTGC | TCCTGCTTGGACATGAAGGCTTT |
qRT-PCR: quantitative real-time polymerase chain reaction, TNF-α: tumor necrosis factor-α, IL-6: interleukin-6, Runx2: runt-related transcription factor 2, ALP: alkaline phosphatase, OCN: osteocalcin.