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. 2022 Feb 28;15(4):405–414. doi: 10.15283/ijsc21152

Table 1.

Sequences of primers used for qRT-PCR

Gene Forward primer sequence (5’–3’) Reverse primer sequence (5’–3’)
β-actin CTACCTCATGAAGATCCTGACC CACAGCTTCTCTTTGATGTCAC
TNF-α ATGTCTCAGCCTCTTCTCATTC GCTTGTCACTCGAATTTTGAGA
IL-6 TCTGGAGCCCACCAAGAACGATAG GTCACCAGCATCAGTCCCAAGAAG
Runx2 CCTTCAAGGTTGTAGCCCTC GGAGTAGTTCTCATCATTCCCG
ALP AACCCAGACACAAGCATTCC CCAGCAAGAAGAAGCCTTTG
OCN GGACCATCTTTCTGCTCACTCTGC TCCTGCTTGGACATGAAGGCTTT

qRT-PCR: quantitative real-time polymerase chain reaction, TNF-α: tumor necrosis factor-α, IL-6: interleukin-6, Runx2: runt-related transcription factor 2, ALP: alkaline phosphatase, OCN: osteocalcin.