Fig. 5.
PTEN overexpression restrains exosomal miR-150-5p-mediated inhibition on H2O2-induced injury in HaCaT cells. (A, B) The expression of PTEN in HaCaT cells transfected with pcDNA3.1 or pcDNA3.1-PTEN by RT-qPCR (A) and western blot analysis (B). (C, D) The expression of PTEN in HaCaT cells by RT-qPCR (C) and western blot analysis (D). (E) Detection of the viability of HaCaT cells by the CCK-8 method. (F) Detection of the migration of HaCaT cells in each group by the plate scratch test. (G) Detection of apoptosis of HaCaT cells in each group by flow cytometry. (H) Detection of the phosphorylation level of PI3K and AKT by western blot analysis. *p<0.05, **p<0.01, ***p<0.001, n=3. The data were presented in the form of mean±standard deviation. The comparison among multiple groups was performed by one-way analysis of variance. Tukey’s multiple tests were used for the post hoc comparison. MSC: mesenchymal stem cells, Exos: exosomes.