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. Author manuscript; available in PMC: 2023 Apr 19.
Published in final edited form as: Sci Transl Med. 2022 Oct 19;14(667):eabh3351. doi: 10.1126/scitranslmed.abh3351

Figure 2.

Figure 2.

Searching synergistic drug combinations with SMAC mimetics or SAHA using CRISPR screening. A, Screening strategy using suboptimal AZD5582 dosage (5 nM). B, Top hits in GFP+ population vs. pooled knockout population. The MAGeCK software is used to calculate the log fold changes and associated p values of each gene. C-D, The significantly enriched pathways associated with depleted (C) or enriched (D) genes in the screens, respectively. Previously reported HIV-1 associated pathways are highlighted in red. The adjusted p values of the top enriched pathways are shown. The enrichment analysis is performed using Enrichr. E, Screening strategy using suboptimal SAHA dosage (0.1 μM). F, Top hits in GFP positive population vs. pooled knockout population. The MAGeCK software is used to calculate the log fold changes and associated p values of each gene.