Fig. 1. Identification of CD45⁺ immune cell populations by unsupervised scRNA-seq clustering in the healthy and inflamed muscularis.

a Experimental pipeline of scRNA-seq experiment. b UMAP of sorted CD45⁺ immune cells from the healthy muscularis, 24 h and 72 h post-injury from WT mice. Each sample was pooled from 3–4 mice. c Heatmap of the 50 most differentially expressed genes in each cluster. d UMAPs of time-dependent infiltration of immune cells upon muscularis inflammation. Data of Fig. 1b has been split based on different time points. e Cell fraction of each cluster relative to the total number of CD45⁺ immune cells at different time points after muscularis inflammation.