Fig. 5. EGCs produce factors essential for the recruitment and differentiation of monocytes during inflammation.

a Relative mRNA levels for Ccl2 and Csf1 normalized to the housekeeping gene rpl32 from ganglia isolated from the muscularis of the small intestine from naïve wild-type mice and 1.5 h, 3 h and 24 h after muscularis inflammation. One-way ANOVA; test *p < 0.05; **p < 0.01; ns not significant. b Immunofluorescent images of muscularis whole-mount preparations at homeostasis and 1.5 h after the induction of muscularis inflammation stained for GFAP (green), HuC/D (gray) and CCL2 (purple). Scale bar (25x) 25 µm, (63x) 15 µm. c Experimental overview of experiments in PLP-CreERT2 Rpl22^HA mice. d Heatmap of HA-enriched differentially expressed genes between immunoprecipitated samples from naïve PLP-CreERT2 Rpl22^HA mice and 3 h after intestinal manipulation. e Selected significant GO terms enriched (GSEA) in PLP1⁺ EGCs 3 h post-injury compared to naive PLP1⁺ EGCs. f Heat map of ligand-target pairs showing regulatory potential scores between top positively correlated prioritized ligands and their target genes among the differentially expressed genes between Ly6c⁺ monocytes and Ccr2⁺ int Mφs. g Circos plot showing top NicheNet ligand-receptor pairs between EGCs and Ly6c⁺ monocytes corresponding to the prioritized ligands in Fig. 5f. h Schematic overview of interactions between EGCs and infiltrating Ly6c⁺ monocytes.