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. 2022 Apr 15;15(6):1257–1269. doi: 10.1038/s41385-022-00513-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — In vitro polarised Th0 and Th2 cells were cultured with αCD3, αCD28 and IL-2 for 4 days with or without IL-10. a Representative histogram of GATA3 staining from IL-10 stimulated and unstimulated Th0 cells. b Percentage of CD4⁺ GATA3⁺ CD4 T cells (left) and concentration (pg/ml) of IL-5 (middle) and IL-13 (right) in Th0 culture supernatants. c Representative histogram of GATA3 staining from IL-10 stimulated and unstimulated Th2 cells. d Percentage of CD4⁺ GATA3⁺ T cells (left) and concentration (pg/ml) of IL-5 (middle) and IL-13 (right) in Th2 culture supernatants. e Percentage of CD44^hi (left) and CD69⁺ (right) CD4 T cells from IL-10 stimulated and unstimulated Th0 cultures. f Representative histograms of Cell Trace Violet staining from IL-10 stimulated (top) and unstimulated (bottom) Th0 cultures. g Proliferation index of IL-10 stimulated and unstimulated Th0 cultures. Graphed data are shown with mean ± 1 SD and are pooled from three independent experiments with n = 4–5 per experiment. Statistical significance was calculated by Student’s t test where data were normally distributed (b, d, e) and Mann–Whitney U test where data were not normally distributed (g). (Significance **p < 0.01, ****p < 0.0001).