Fig. 6. IL-10 signalling promotes the intestinal Th2 response in H. polygyrus infection.

C57BL/6 mice were infected with 200 L3 H. polygyrus and at D-1, D2 and D5 treated with anti-IL-10R mAb or isotype control, and 7 days post-infection the small intestine and MLN collected for analysis. a Representative H&E staining of the duodenum from naïve (top left), D7 infected + isotype control parasite area (bottom left), D7 infected + isotype control distal area (top right) and D7 infected + anti-IL-10R mAb parasite area (bottom right). Histology scoring of (b) inflammation depth and (c) combined inflammation score from the three treatment groups. d Percentage of GATA3⁺ (top) IL-13⁺ (middle) IL-5⁺ (bottom) of CD4⁺ CD44^hi T cells in the MLN. e Percentage of GATA3⁺ (top) IL-13⁺ (middle) IL-5⁺ (bottom) of CD4⁺ CD44^hi T cells in the SILP. Representative staining in the SILP of GATA3 (top), IL-13 (middle) and IL-5 (bottom) from H. polygyrus infected (f anti-IL-10R treated, g isotype control treated). Graphed data are shown with mean ± 1 SD and are pooled from 2–3 independent experiments with n = 4–5 per experiment. Statistical significance was calculated by ANOVA followed by a Tukey’s post-test for multiple comparisons between groups where data were normally distributed (d (IL-5, IL-13) and e (GATA3)) and Kruskal–Wallis test with Dunn’s post-test for multiple comparisons between groups where data were not normally distributed (d (GATA3), e (IL-5, IL-13). (Significance *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).