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. 2022 Nov 28;12:20518. doi: 10.1038/s41598-022-25118-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Column representation of visceral viral loads and a pathological scores of rabbits infected with GI.1. The viral load was calculated according to the formula 2^−ΔΔCT. The higher the ΔCt value is, the lower the 2^−ΔΔCT value is. The heart with the highest ΔCt value and the lowest expression of GI.1 was selected as Calibrator. Different asterisks indicate significant differences, *(p < 0.05), **(p < 0.01), ***(p < 0.001), ****(p < 0.0001); ns means the difference is not significant (p > 0.05).