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The sequences of primer.
| Primers | Primers sequence (5′–3′) | Target gene | Annealing temperature (°C) | Fragment length |
|---|---|---|---|---|
| GI.2-F | AACCACTGCCGGTGACAG | VP60 | 55.4 | 203 |
| GI.2-R | ATCAACACTCAAGCCAAG | |||
| GI.1-F | TGGAGATYGGTTTRAGTG | VP60 | 57.1 | 165 |
| GI.1-R | AATGAGTTCAGTCARGTCAA | |||
| β-actin-F | CAAGCGTGGCATCCTGAC | β-actin | 60 | 100 |
| β-actin-R | CTCGTTGTAGAAGGTGTGGTG |
