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. 2022 Nov 28;13:7199. doi: 10.1038/s41467-022-34863-9

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© The Author(s) 2022, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Single cells derived from 3 WT (left panel) and 3 littermate-matched Lats1-CKO (right panel) PyMT-driven tumors were processed for EpiTOF analysis as described in the “Methods” section. Shown is a uniform manifold approximation and projection for dimension reduction (UMAP). Relevant populations are indicated by circles. LumP luminal progenitors. b Comparison of the relative levels of EpiTOF markers in the WT vs. Lats1-CKO EpiTOF-defined in vitro basal-like subpopulations. Expression of each of the markers was centered to zero mean and displayed as a split violin plot ordered by directionality and extent of the difference. Black lines designate medians. Red boxes denote H3K36me2 and H3K27ac. c Violin plot comparing mean expression levels of the indicated epigenetic marks in WT vs. Lats1-CKO PyMT cells in the three different subpopulations of the in vitro EpiTOF samples (defined in a). d Western blot analysis of H3K27ac and H3K36me2 in WT and Lats1-CKO in vitro samples. GAPDH served as a loading control. Total H3 levels are also shown. Representative blot of five biological repeats. e Bivariate contour plots showing the expression (Z-scores) of EpCAM and CD49f in WT vs. Lats1-CKO in vitro EpiTOF samples, within the indicated subpopulations.