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. 2022 Nov 28;13:7199. doi: 10.1038/s41467-022-34863-9

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© The Author(s) 2022, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a MYC-tagged mouse Lats1 or vector control was stably introduced into three independent Lats1-CKO cell lines. Left: representative FACS analysis as in Fig. 3a. Right: graphical representation of the proportional increase in luminal cells in each cell line upon LATS1 overexpression, measured as in (a, left) (mean ± SD of 3 cell lines). Source data are provided as a Source Data file. b Lats1-CKO PyMT cells stably harboring vector or MYC-tagged mouse Lats1 were subjected to Western blot analysis with the indicated antibodies. A WT sample is presented in the first lane for comparison. GAPDH served as a loading control. Numbers under lanes represent relative H3K27ac band intensity, normalized to the corresponding loading control and control sample. Representative blot of five biological repeats. c MCF10A cells, either WT or with CRISPR/Cas9 deletion of LATS1 (LATS1-KO), were subjected to Western blot analysis with the indicated antibodies. Tubulin served as a loading control. Numbers are as in (b). Representative blot of two biological repeats. d MCF7 cells transiently transfected with the indicated siRNAs were subjected to Western blot analysis with the indicated antibodies. GAPDH served as a loading control. Numbers are as in (b). Representative blot of three biological repeats. e MDA-MB-468 cells harboring vector control or human MYC-LATS1, after 48 h of doxycycline induction, were subjected to Western blot analysis with the indicated antibodies. Top panel = 9E10 antibody, directed against the MYC-tag. GAPDH served as a loading control. Numbers are as in (b). Representative blot of four biological repeats. f GSEA of MCF7 (left) and ZR-751 (right) cells, transiently transfected with control siRNA (siCont) or LATS1 siRNA (siLATS1) (data from Furth et al.⁶¹), compared to an ER repressed³⁰ gene set. g FACS analysis of MDA-MB-468 cells harboring vector control or human MYC-LATS1, after 48 h of doxycycline induction. Cells were first stained for APC-EpCAM, and then permeabilized to stain intracellular LATS1 (probed with Alexa Fluor 488 dyed secondary antibody). Black square designates the quadrant of EpCAM^high luminal cells. h GSEA of MDA-MB-468 cells without (vector) vs. with induction of LATS1 (OE-LATS1), compared to genes upregulated in human luminal tumors relative to basal-like tumors (data from TCGA).