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. 2022 Nov 28;13:7199. doi: 10.1038/s41467-022-34863-9

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© The Author(s) 2022, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Chromatin was immunoprecipitated from WT or Lats1-CKO PyMT cells using antibodies against endogenous NCOR1, followed by qPCR analysis of regulatory regions of the indicated LATS1–NCOR1-repressed genes. Values were normalized to input. Beads without antibodies, incubated with chromatin, served as background control. Values represent the average of two biological replicates. Source data are provided as a Source Data file. b WT or Lats1-CKO PyMT cells were transfected with control siRNA (siC) or siRNA against Ncor1 (siN) for 48 h. Chromatin was immunoprecipitated using antibodies against H3K27ac, followed by qPCR analysis of regulatory regions of the indicated LATS1–NCOR1- repressed genes. Analysis was as in (a). Average of two biological replicates. Source data are provided as a Source Data file. c WT and Lats1-CKO cells were infected with recombinant lentiviruses expressing control shRNA (shCont) or shRNA against Ncor1 (shNcor1) and maintained under selection for at least 2 weeks. Representative FACS profiles are presented (left). A graphical representation of the relative portions of luminal (high EpCAM) and basal-like (low EpCAM) cells is shown on the right (mean ± SE of three biological replicates). One-way ANOVA was used to calculate significance. Source data are provided as a Source Data file.