Table 3.

Comparison between five-plex and simplex RT-ddPCR.

Viral titera	AMPV-A			AMPV-B			AMPV-C			AMPV-D
	Five-plexb	Simplexb	Bias	Five-plexb	Simplexb	Bias	Five-plexb	Simplexb	Bias	Five-plexb	Simplexb	Bias
4	3.27	3.46	5.49	2.87	3.10	7.42	2.79	2.85	2.11	2.57	2.90	11.38
3	2.27	2.48	8.47	1.84	2.03	9.36	1.86	1.89	1.59	1.60	1.89	15.34
2	1.24	1.46	15.07	0.91	1.12	18.75	0.89	0.85	−4.71	0.61	0.92	33.70
1	0.44	0.41	−7.32

^aLog₁₀ (TCID₅₀/ml).

^bLog₁₀ (absolute copies/μl of extracted viral RNA), Bias: expressed in % [calculation: 100–(five-plex result/Simplex result^*100)].