Table 5.

Limits of detection and quantification.

Sub-group	Viral titera	Assay 1b			Assay 2b			Copies/μl	R
		Rep 1	Rep 2	Rep 3	Rep 1	Rep 2	Mean +/– SD (CV)
A	4	3.27	3.25	3.39	3.23	3.21	3.27 +/– 0.07 (2.2)		0.9971
	3	2.32	2.28	2.30	2.25	2.18	2.27 +/– 0.05 (2.4)
	2	1.26	1.28	1.25	1.16	1.25	1.24 +/– 0.05 (3.9)
	1	0 .46	0 .53	0 .51	0 .36	0 .32	0.44 ±/– 0.09 (20.8)	3
	0	N	N	N	N	N
B	4	2.83	2.87	2.89	2.87	2.89	2.87 +/– 0.03 (0.9)		0.9990
	3	1.81	1.80	1.81	1.84	1.92	1.84 +/– 0.05 (2.6)
	2	0.88	0.89	0.94	0.94	0.91	0.91±/–0.03 (3.4)	8
	1	0.26	0.36	0.18	0.15	0.40	0.27 +/– 0.11 (41.4)	2
	0	N	N	N	N	N
C	4	2.84	2.82	2.70	2.79	2.79	2.79 +/– 0.05 (1.9)		0.9999
	3	1.84	1.91	1.85	1.85	1.83	1.86 +/– 0.03 (1.7)
	2	0.81	1.00	0.78	0.90	0.94	0.89±/–0.09 (10.3)	8
	1	−0.10	−0.22	−0.30	−0.10	−0.40	−0.22 +/−0.13 (58.7)	1
	0	N	N	N	N	N
D	4	2.57	2.56	2.57	2.58	2.58	2.57 +/– 0.01 (0.3)		1.0000
	3	1.55	1.58	1.62	1.59	1.65	1.60 +/– 0.04 (2.3)
	2	0.51	0.67	0.59	0.67	0.59	0.61±/–0.07 (11.5)	4
	1	−0.15	0.15	0.15	−0.15	0.04	0 +/– 0.15	1
	0	N	N	N	N	N

^a: Log₁₀ (TCID₅₀/ml).

^b: Log₁₀ (absolute copies/μl of extracted viral RNA), R², Co-efficient of determination of the linear regression curve; Rep, replicate number; SD, standard deviation; CV, co-efficient of variation expressed in %; N, not detected; bold line: limit of detection; underlined line: limit of quantification.