Table 2.

LC–MS–MS results for MpeV co-expressions. Extracted ion chromatograms were made using m/z 1200.8254 for 38–78 peptides with a bilin attached; 1053.7513 was extracted for the 38–78 peptide lacking a bilin. Sample abbreviations are defined in Supplementary Table 1.

Sample	38–78 peptide cysteine statea	Retention timed	EIC areab
WHCpeBA + RSCpeZ/CpeS, no MpeV	2B	N/D	N/D
	1B	N/D	N/D
	0B	22.34	3.34E+08
WHCpeBA + RSCpeZ/CpeS + WHMpeV	2B	20.94	2.35E+07
	1B	N/D	N/D
	0B	N/D	N/D
WHCpeBA + RSCpeZ/CpeS+ RSMpeV	2B	21.19	7.73E+08
	1B	N/D	N/D
	0B	22.6	1.08E+08
RSCpeBA + RSCpeZ/CpeS + WHMpeVc	2B	25.71,26.44	4.57E+07
	1B	27.25	3.59E+07
	0B	27.23	2.32E+07
WHCpeB(H141L)A + RSCpeZ/CpeS + RSMpeVc	2B	21.14	4.42E+09
	1B	23.2	6.79E+07
	0B	22.54	8.07E+07
WHCpeB(H141L)A + RSCpeZ/CpeS + WHMpeVc	2B	25.83,26.48	2.32E+08
	1B	27.27	2.41E+07
	0B	21.97	7.82E+07

Representative tandem mass spectra for each form of the peptide can be found in Supplementary Figures 5–7.

^aThe 38–78 peptide has C50, C61 and C73. Peptides with doubly-linked bilin bridging C50 and C61 are labeled 2B. Peptides with a singly-linked bilin at C50 are labeled 1B; peptides with no bilin but a disulfide between C60 and C73 are labeled 0B.

^bAll EICs were made using an 8 ppm mass window and a 3 point boxcar smoothing routine. Areas were integrated with the ICIS algorithm of Thermo QualBrowser 4.3.73.11. Results are representative of two independent replicates.

^cSample was run separately from the others using a slightly modified gradient.

^dN/D means not detected.