FIG. 1.

Binding affinities of the peptides for S. aureus LTA as measured by the DPX displacement assay. Purified soluble S. aureus LTA (5 μg/ml) was incubated with 2.5 μM DPX, and the fluorescence at 485 nm was measured. Peptides were added in increments of 5 μg/ml, and the DPX fluorescence was measured after each addition. Each data point represents the mean of values from three independent experiments. The standard errors of the means were all less than 10%. PB, polymyxin B.