FIG. 5.

Effects of anti-CTLA4 treatment on cytokine mRNA expression in the draining lymph nodes of BALB/c and IL-4 KO BALB/c mice at 4 weeks of infection. Groups of four BALB/c and IL-4 KO mice each were infected with L. major as described above. Where indicated, IL-4 KO mice were injected with saline or 0.5 mg of anti-CTLA4 MAb on days 0 and 7 of infection. After 4 weeks, lymph nodes were harvested into STAT-60 and RNA obtained for reverse transcriptase PCR analysis. Shown are the results of comparative reverse transcriptase PCR analysis for the indicated cytokine mRNA as measured in arbitrary densitometry units that are corrected for hypoxanthine phosphoribosyl transferase (HPRT) expression. Expression of these mRNA was not detected in uninfected lymph nodes at the levels of amplification used.