TABLE 3.
IL-4-independent effects of anti-CTLA4 antibody on cutaneous parasite loada
| Expt and mouse strain | Treatment | Parasite load (log10 ± SEM) | P valueb |
|---|---|---|---|
| Expt 1 | |||
| BALB/c | Saline | 5.54 ± 0.46 | |
| BALB/c | Anti-CTLA4 | 7.33 ± 0.29 | 0.02 |
| BALB/c | Anti-IL-4 | 3.15 ± 0.19 | <0.001 |
| BALB/c | Anti-CTLA4 plus anti-IL-4 | 6.99 ± 0.40 | 0.05 |
| Expt 2 | |||
| BALB/c | Saline | 9.20 ± 0.17 | |
| BALB/c IL-4 KO | Saline | 6.75 ± 0.18 | 0.005 |
| BALB/c IL-4 KO | Anti-CTLA4 | 8.53 ± 0.46 | 0.25 (0.02) |
a
Shown are the logarithmic mean numbers ± the SEM of parasites per gram of tissue as determined by limiting dilution culture for leishmania. Mice were infected for 4 weeks with 2 × 106 promastigotes of L. major. Where indicated, mice were treated with 0.3 mg of anti-CTLA4 MAb on days 0 and 7 of infection or with 1 mg of neutralizing anti-IL-4 MAb 11B11 on day 0.
b
P values were determined by use of the Student's t test (n = five mice each) and compare the indicated experimental groups with their respective BALB/c controls. Parentheses in experiment 2 indicate significantly increased parasite loads in anti-CTLA4-treated IL-4 KO mice compared to IL-4 KO controls.