Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Nov 28;13:7332. doi: 10.1038/s41467-022-34796-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — During La-dependent processing (top, left) in previously studied eukaryotes such as yeast, the La protein is the first protein to associate with pre-tRNAs on the 3’-stretch of uridylates generated by RNA polymerase III transcription termination. Binding of La provides protection from degradation by 3’-exonucleases, assists with tRNA folding through RNA chaperone activity and stabilizes the nascent pre-tRNA. The next step in tRNA processing is endonucleolytic removal of the 5’-leader by RNase P, followed by an endonucleolytic cut by RNase Z resulting in removal of the 3-trailer sequence and the La protein bound to the uridylate stretch. In contrast, during La-independent processing of pre-tRNAs (top, right), the 3’-trailer is rapidly removed first by 3’-exonucleases such as Rex1p, followed by RNase P processing resulting in an end-matured tRNA. Our data from T. thermophila point towards a pre-tRNA processing model in which Mlp1-dependent processing (bottom, left) 3’-trailers are processed efficiently. Mlp1-independent processing results in the accumulation of pre-tRNAs containing unprocessed 3’-trailer sequences, indicating that Mlp1 is required for efficient 3’-end processing unlike other eukaryotes. Image created with BioRender.com. The Publication License is provided as a Source Data file.