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. 2022 Nov 28;12:20503. doi: 10.1038/s41598-022-23690-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Transconjugant numbers and calculated conjugation frequencies, observed at different concentrations of ampicillin (a,b), Zn(NO₃)₂ (c,d), and rifampicin (e,f). Pairwise comparison of transconjugant numbers and conjugation frequencies at different concentrations was perform via the Student’s T-test. P-values were adjusted using the Bonferroni correction. Significance levels: *p < 0.05, **p < 0.01, ***p < 0.001. Boxplot in brackets (f) indicates that the calculation of conjugation frequencies for some data points was omitted due to no observed transconjugants.