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. 2022 Nov 28;12:20503. doi: 10.1038/s41598-022-23690-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Results of the WGS analysis via ABRicate, represented in the form of heatmaps, showing (a) comparison of observed plasmids and (b) antimicrobial resistance genes (highlighted in red) between donor, rif^R recipients, and transconjugants of the conjugation assay with Zn(NO₃)₂. (c) Reconstruction of the transferred plasmid from donor to recipients (transposases highlighted in green; genes involved in plasmid replication highlighted in purple; genes involved in AMR highlighted in red).