Table 1.

Pharmacological stimuli.

Compound	Target	Mechanism	Specificity	Source	Vehicle	Conc.	Incubation time (h)	Ref.
MG132	Proteasome	Binds the ß5 20 S subunit	+++	Sigma #C2211-5MG	DMSO	10 µM	18 h	53,54
VER155088	HSP70	Binds the ATPase domain	+++	Sigma #SML0271	DMSO	20 µM	18 h	8,55
Staurosporine	Kinases	Non-selective ATP-competitive inhibitor	++	AbCam #ab146588	DMSO	500 nM	2 h	56,57
Celastrol	Heat shock response	Activation of HSF1 transcriptional regulation	+	Sigma #0869	DMSO	5 µM	18 h	22,58,59
Novobiocin	HSP90	Binds the ATP-binding site of the ATPase subunit	+	Sigma #N1628	mQ	800 µM	6 h	26,55

Compounds were diluted into fresh culture media before incubation at 37 °C. Compounds are categorized as poor (+), moderate (++), and high (+++) specificity according to the scope of the target and reported range of off-target effects.