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. 2022 Nov 15;13:974377. doi: 10.3389/fimmu.2022.974377

Figure 10.

Figure 10

CPEB3 inhibits cell proliferation, colony formation, and invasion in HCC cells. (A) The CPEB3 abundance in normal liver cells and HCC cells at protein and mRNA levels. (B) Hep3B cell was stably transfected with LV-shNC and LV-shCPEB3. The expression of CPEB3 was detected using qRT-PCR and Western blot. (C) Hep3B cells were stably transfected with LV-ctrl and LV-CPEB3. The expression of CPEB3 in stably overexpressed and control Hep3B cells was detected using qRT-PCR and Western blot. (D) Cell Counting Kit-8 was used to quantify the proliferation of Hep3B-Ctrl, Hep3B-shCPEB3, and Hep3B-CPEB3. (E) Colonies of Hep3B-Ctrl, Hep3B-shCPEB3, and Hep3B-CPEB3 were quantified following continuous incubation for 14 days. (F) Transwell assays were carried out to determine the effect of CPEB3 on the cell invasion. *P < 0.05; **P < 0.01; ***P < 0.001.